Altered peripheral T-cell function in p110γ^KOδ^D910A mice. (A-B) Purified T cells were cultured with purified and mitomycin C–treated T-cell–depleted splenocytes from WT (B6) or SJL mice for 48 hours. ³H-thymidine (TdR) was added at 42 hours. (C-H) Purified T cells from lymph nodes of WT or p110γ^KOδ^D910A mice were cultured with plate-coated α-CD3 (at indicated concentrations, μg/mL) with or without α-CD28 (10 μg/mL) for 48 hours. Proliferation was measured as in panel A and cytokines from supernatant were measured by cytometric bead arrays. Data represent at least 2 experiments with triplicates for each sample (± SEM).