Lymphopenia in p110γ^KOδ^D910A mice. (A) H&E staining of spleen (4×/0.12 objective lens). (B-E) Flow cytometry analysis. (B) B220 and CD3 profile of spleen. (C) CD4 and CD8 profile of spleen. (D) Numbers of B220⁺, CD4⁺, and CD8⁺ cells from spleen and lymph nodes. (E) CD44 profile gated on CD4⁺ cells from spleen. Data are from 6 mice in each group (± SEM). Numbers inside panels B and C indicate percentage of gated cells in total splenocytes; numbers in panel E, percentage of gated cells in CD4⁺ splenocytes.