Combined effect of CsA and INNO-406 in an in vivo CNS leukemia model. BALB/cA Jcl-nu mice were inoculated into the right cerebral ventricle with 5 × 10⁴ BaF3/wt bcr-abl^GFP cells on day 0. Brains from each group were taken on day 17 from mice given vehicle (A), 50 mg CsA/kg (B), 60 mg INNO-406/kg per day (C), or a combination of 60 mg INNO-406/kg per day and 50 mg CsA/kg (D). On day 17, 3 mice from each group were killed, and their brains were examined under a fluorescence stereoscopic microscope. Kaplan-Meier analysis of the in vivo effects of INNO-406, CsA, or both on wt BCR-ABL (E) and BCR-ABL/M351T (F). Mice were orally administered vehicle (black), 50 mg CsA/kg (blue), 60 mg INNO-406/kg per day (pink), or a combination of CsA and INNO-406 (red) from day 5 to day 15.