Detection of anti–HIV-1 antibodies from the plasma of HIV-1–infected mice. An ELISA assay was conducted by using plasma from 14 mice inoculated with either HIV-1_JRCSF or HIV-1_MNp, and from 2 uninfected control mice. Representatives (n = 8) of the 14 HIV-1–inoculated mice, and the 2 uninfected mice, are shown in the panels. Measurements of specific human antibodies for HIV-1_IIIB gp120 (A), HIV-1_MN gp120 (B), and HIV-1_IIIB p24 antigens (C) were shown. Results are expressed as the means from triplicate assays in 3 different experiments.