Activation of the NFκB pathway induces a notch signal in neighboring cells. ECs were transfected with an expression plasmid for CA-IKKβ, and induction of jagged was confirmed by FACS (data not shown). Cells were then cocultured with ECs transfected with the notch reporter plasmid RBP-Luc, in the presence or absence of the notch signaling inhibitor DAPT (gamma-secretase inhibitor). Cells were harvested at 18 hours for assay of luciferase activity. Mean and SD is shown. *P < .01 by Student t test. One of 2 similar experiments.