The FMNL1-PP2–specific T-cell clone shows high functional activity and an intermediate functional avidity. (A) T2 cells pulsed with FMNL1-PP2 (10 μM) were used as targets for the FMNL1-PP2–specific T-cell clone at different effector-target ratios in a ⁵¹Cr-release assay. Error bars indicate the standard deviation of tested duplicates. (B) T2 cells were pulsed with FMNL1-PP2 at different concentrations and used as targets for the FMNL1-PP2–specific T-cell clone in a ⁵¹Cr-release assay at an effector-target ratio of 7.5:1. Tyrosinase- and Her2/neu-derived peptides were used as negative controls. Error bars indicate the standard deviation of tested duplicates.