Images of real-time series of fibrin network formation. The images of a low-magnification time series of micrographs were thresholded and colorized as described in “Establishment of the scaffold and network formation.” Original images from a similar time course of polymerization are shown in Videos S1,S2. (A) First fibers appear, corresponding to the lag period of the turbidity curve. (B) The scaffold appears, corresponding to the time when the turbidity curve starts increasing. (C) Corresponds to the gelation time measured by eye. (D) Network is fully formed, corresponding to the time when the turbidity curve reached a plateau. (E) Represents merged panels A and B, where the panel A was colored red, and panel B blue. (F) Represents merged panels B and C, where panel B was colored red, and panel C blue. Fibers that have purple color (mixture of red and blue) represent the same fibers at the 2 time points. Fibers that have blue color represent newly formed fibers. Fibers that have red color are the fibers that changed position or were rearranged. (G) Represents merged panels C and D, where panel C was colored red, and panel D was blue. Purple fibers are the same fibers at the same positions, red fibers are rearranged fibers, and blue fibers are newly formed fibers. (H) Turbidity curve with marked time points A, B, C, D, each corresponding to the time point represented in panels A through D, respectively. Bar represents 40μm.