Series of micrographs from the deconvolution microscope showing the dynamics of fibrin network formation. Fibrin was labeled with Alexa 488 as described in “Labeling of fibrinogen with Alexa 488.” Images were taken every 10 seconds (Videos S1,S2, available on the Blood website; see the Supplemental Materials link at the top of the online article). Selected images at the following times are shown: (A) 3 minutes and 50 seconds, (B) 4 minutes, (C) 4 minutes and 10 seconds, (D) 4 minutes and 20 seconds, (E) 4 minutes and 40 seconds, (F) 5 minutes, (G) 6 minutes, and (H) 20 minutes. Arrowheads () indicate new fibers. Thick arrows (↙) indicate fibers that change position. Thin arrows (↑) indicate fibers that change length. The circled area shows a single-fiber with a branch point that is not connected to the scaffold in panel D, but it has connected to the scaffold later, as in panel E. Bar represents 15 μm.