LRP-1 deletion in macrophages in vivo increases expression of C1r and iNOS. (A) PEA-10 and MEF-2 cells were transfected with pNF-κB-Luc to measure NF-κB activity. Cells were treated with TNF-α-neutralizing antibody or control IgG for 18 hours. Luciferase activity then was measured. (B) PEA-10 and MEF-2 cells were transfected with pNF-κB-Luc. Cells were treated with LPS (100 ng/mL) or vehicle. Luciferase activity was measured 6 hours later. (C) Macrophages were harvested from the IP space of mice in which LRP-1 was conditionally deleted in macrophages and neutrophils and from control wild-type mice in the same genetic background. RNA was isolated from peritoneal cells 3 days after thioglycolate stimulation. Expression of LRP-1, C1r, and iNOS was determined by qPCR. (D) Peritoneal macrophages were cultured for 12 hours in the presence of JSH-23 (10 μM) or vehicle. Expression of EGFr, uPAR, and MCP-1 was analyzed by qPCR. Error bars represent SD.