CD3⁺CD4^low and CD3⁺CD8^low suppressor T cells are overrepresented in patients who underwent transplantation with high plasma levels of HLA-G, IL-10, and sCD4/sCD8 molecules. (A-B) Soluble HLA-G levels were measured in plasma from healthy donors (HDs, n = 14), and patients who received a kidney transplant (KT, n = 34), liver transplant (LT, n = 21), and combined liver-kidney transplant (LKT, n = 44). Concentrations were measured by ELISA using as capture antibody either (A) MEM-G/9 specific for both shed HLA-G1 and HLA-G5 or (B) 5A6G7 reacting only with HLA-G5 and as detection antibody either (A) anti-β2m or (B) W6/32. (C) PBMCs from HD, KT, LT, and LKT were analyzed by 3-color flow cytometry for surface expression of CD3, CD4, and CD8. Results are shown as dot plots in which the low and high coreceptor-expressing cells were defined according to the basal expression level observed in healthy donors. Numbers represent the percentage of cells within the corresponding region. Six individuals from each group were analyzed showing a similar pattern of CD4 and CD8 coreceptor expression. One representative pattern is shown. (D-E) Levels of soluble CD4 and CD8 molecules were measured by specific ELISA in plasma from KT, LT, and LKT patients. (F) IL-10 and IL-2 concentrations were measured by ELISA in plasma from KT, LT, and LKT patients. (G) Comparison of reactivation posttransplantation of viral infections due to hepatitis C virus and sHLA-G plasma levels is shown for LT patients dividing according to the occurrence of HCV relapse or not (HCV neg).