CD3⁺CD4^low and CD3⁺CD8^low T cells are induced after APC-HLA-G1 priming. (A) Morphology and granularity of T cells primed with either APCs or APC-HLA-G1s are provided on a forward scatter (size) and side scatter (granularity) graph. APC-primed T cells are enlarged (mean fluorescence intensity = MFI, X-MFI = 750) and granulated (Y-MFI = 62), while APC-HLA-G1–primed T cells are smaller (X-MFI = 590) with fewer granularities (Y-MFI = 47). (B,C) T cells primed with either APCs (left panel) or APC-HLA-G1s (right panel) were analyzed for surface expression of CD3, CD4, and CD8 at day 6 of MLR using 4-color flow cytometry. Results are expressed as (B) dot plots gated on CD3⁺ T cells or (C) histograms. (B) X-MFI corresponds to CD3-MFI, and Y-MFI indicates CD4-MFI or CD8-MFI. (C) Numbers on the left of each graph represent the percentage of positive cells (top) and the MFI calculated on the positive cells (bottom). Two representative experiments of 6 are shown.