HLA-G–induced CD4⁺ and CD8⁺ T cells are Foxp3⁻ and their expression of CD18, CD25, CD28, CD122, CD137, CD152, CD154, GITR, and PD1 is not modulated. (A,B) T cells were primed with either APCs or APC-HLA-G1s and were analyzed by 4-color flow cytometry at day 6 of MLR for expression of PD1, GITR, CD25, CD122, CD152, CD137, CD154, CD28, and CD18 among the CD3⁺CD4⁺ (A) and CD3⁺CD8⁺ (B) T cells. Results are shown as histograms representing the percentage of positive cells. One representative experiment of 5 is shown. (C) Intracellular Foxp3 protein expression was analyzed by flow cytometry in T cells primed with either APCs or APC-HLA-G1s at day 6 of MLR. Analysis was done after cell permeabilization on CD3⁺-gated cells. Positive control of Foxp3 staining was provided by anti-CD3/anti-CD28 activated T cells. Permeabilization was assessed by α-tubulin staining. One representative experiment of 3 is shown.