Adoptive transfer of WT but not MyD88-deficient eosinophils significantly accelerates viral clearance and suppresses RSV-induced AHR. (A) Experimental design. (B,C) Eosinophils were isolated from the spleens of hypereosinophilic MyD88-sufficient or -deficient mice and sorted by FACS. WT or MyD88^−/− eosinophils were instilled directly into the trachea 2 hours before inoculation with RSV into WT (B) or MyD88-deficient (C) mice, and virus clearance was assessed at 6 dpi (D,E). Mice were treated as described in panels B,C and assessed for alterations of airway responsiveness to methacholine (50 mg/mL, dose that gives maximal responsiveness) by whole-body plethysmography. Data represent the mean plus or minus SEM (n = 3-4 mice per experimental group). *P < .05; **P < .01; #P < .05; ##P < .01.