Lineage conversion ability of IL-2Rβ is transferable to IL-7Rα. (A) Diagram of chimeric receptors that are composed of IL-7Rα and subdomains of the IL-2Rβ cytoplasmic tail. The A region of IL-2Rβ was fused to the C terminus of IL-7Rα, resulting in 7α/7α/7α-2βA. Addition of the A region of IL-2Rβ to IL-7Rα enables lineage conversion activity of IL-7R as judged by differentiation readout on (B) OP9 stromal cell cultures and (C) methylcellulose cultures. (B) Percentages indicate total cells in the gates as described in Figure 1C. (C) Error bars represent standard deviation from triplicate samples.