BCR/ABL posttranslationally enhances hnRNP-E2 expression by increasing its protein stability. (A) Western blot (left panel) and RT-PCR (right panel) analyses of hnRNP-E2 expression in parental and BCR/ABL-transduced 32Dcl3 cells cultured in the presence of IL-3 or IL-3 deprived for 8 hours. (B) Effect of ALLN (25 μM) and ALLM (25 μM) on hnRNP-E2 levels. Note that 100 μg and 25 μg lysates from 32Dcl3 and 32D-BCR/ABL cells were loaded for each lane, respectively. (C) Effect of the protein synthesis inhibitor cycloheximide (CHX) on hnRNP-E2 and hnRNP-E1 levels in parental and BCR/ABL-transduced 32Dcl3 cells.