Absolute lymphocyte reconstitution. (A-E) Scatterplots indicate absolute cell numbers in patients and donors or (F) percentage of donor T-cell chimerism in patients; horizontal bars represent the median of each group. Red circles refer to patients with clinically significant (≥ grade II) aGvHD development. The gray-shaded area represents the interquartile (25%-75%) range of cell counts in donors. In panels A through E, ALC indicates absolute lymphocyte count (as determined by the department of laboratory medicine by flow analysis and lymphocyte gating, includes T and B lymphocytes, NK cells, and some monocytes). After immunophenotyping of frozen cell specimen, absolute numbers for CD3⁺, CD3⁺ CD4⁺, CD3⁺ CD8⁺ (determined as CD3⁺ CD4⁻) and CD3⁻ (calculated by subtraction of CD3⁺ from ALC) cells were calculated from ALCs. Pre indicates patients before transplantation (n = 11); Don, donor (n = 13); d30, d60, and d90, patients 30 (n = 13), 60 (n = 8), and 90 (n = 5) days after transplantation, respectively. In panel F, donor T-cell chimerism in selected CD3⁺ cells: d30 (n = 13), d60 (n = 7), and d90 (n = 5).