In vitro migration of mobilized CFU, KL and SKL cells. (A) Mobilized PBMCs were depleted of lin⁺ cells, stained with FITC-conjugated anti-c-kit and c-kit⁺ lin⁻ cells (shown in R1) isolated by FACS, and subjected to in vitro migration to 100 ng/mL of rmSDF-1α. (B) Percent CFU-GM or GEMM migration in KL mobilized cells in response to recombinant murine SDF-1α. Data are expressed as mean (± SEM) from 2 experiments with 3 mice per group. *P < .05 compared with G-CSF. (C) Migration of CD34-SKL cells in mobilized peripheral blood in response to 100 ng/mL recombinant murine SDF-1α. Data are expressed as mean (± SEM) from 2 experiments with 20 mice per group. *P < .05 compared with G-CSF. (D) Expression of cell surface (left) and intracellular CXCR4 (right) for SKL cells mobilized by G-CSF and GROβ_Δ4. The insert shows one representative plot; the bar graph represents mean (± SEM) from 3 replicate experiments with 10 mice per group.