MN1 immortalizes murine BM cells and induces AML in mice. (A) Western blot using an antihuman MN1 and antimouse Gapdh antibody in lysates of control (ctl)- and MN1-transduced BM cells. (B) Total number of GMPs in serial replating. Cumulative yield is shown for an initial plating of 4000 transduced BM cells (mean ± SD of 3 [MN1] or 2 [control] independent experiments). (C) Survival curves for mice that received transplants of MN1-transduced (n = 18, 5 independent experiments) or GFP control-transduced (n = 7, 3 independent experiments) BM cells. (D) Representative Wright-Giemsa–stained cytospin preparation of BM cells from a leukemic mouse (MN1). (E,F) Representative hematoxylin-eosin–stained tissue sections from spleen (E) and liver (F) of leukemic mice (MN1). See “Materials and methods” for image acquisition information. (G) Immunophenotype of leukemic BM cells at time of death gated on GFP⁺ cells (mean ± SD of 9 analyzed mice). (H) Southern blot analysis using 10 μg genomic DNA of bulk BM from leukemic MN1 mice (4 mice each of 2 independent BM transductions; panels B,C). The cDNA for eGFP was used for probing.