HLA-G^pos T cells are present in the human thymus in mature CD4 and CD8 subsets. FACS analysis of HLA-G expression in thymocyte subsets: Thymocytes were stained for CD4, CD8 and HLA-G. Gates were set on CD4⁺CD8⁻ and CD4⁻CD8⁺ single-positive thymocytes as well as on double-positive thymocytes. The percentage of HLA-G^pos cells was assessed in comparison with a negative control. The summary of 6 independent stainings is shown. Horizontal bars indicate the mean of 6 samples; vertical bars show SEM.