GT4 and GT2-specific peptide variants representing autologous viral sequence of patients C29 and C35 are not cross-recognized in patients with resolved GT1 infection. HCV-specific cell lines of 2 patients with spontaneously resolved HCV infection (serologic GT1 according to Murex serotyping test: “Patients, materials, and methods”) were tested by dilution ICS for IFN-γ production for their responses against GT1 peptides (■) and autologous peptides (▲) of non-GT1 patients C29 and C35. (A) spontaneous resolver patient R23 shared the restricting HLA DR1*1 alleles for epitope p158 (aa1581-1600) that was detected in patient C35. The GT1-specific peptdie sequence but not the GT2b-specific autologous variant of the non-GT1 patient was recognized in dilution ICS experiments (“Patients, materials, and methods”). (B) Spontaneous resolver patient R1 shared the restricting HLA allele DRB1*11 for the previously described epitope P227 (aa2271-2290) that was detected in non-GT1 patient C29. Here also, the GT1 peptide sequence, but not the GT4-specific autologous variant of non-GT1 patient C29 was recognized.