Synthesis and secretion of mouse ADAMTS13. Mouse ADAMTS13 cDNA constructs were used to transfect HEK 293T cells. The synthesized proteins were separated by SDS-PAGE and visualized by immunoblotting with ant-V5 tag sequence. A representative composite gel of ADAMTS13 proteins in the cell lysates and culture media for each cDNA. The cell lysates were diluted by 2-fold for the analysis.