iDC^Zol+ induce the expansion of CM and EM γδ T cells with specific homing and costimulatory receptors. (A) Representative dot plots of γδ T-cell subset distribution after 7 days stimulation with iDC^Zol− and iDC^Zol+. Four subsets are identified according to the expression of cell surface CD45RA and CD27 antigens after backgating on γδ T cells: naive (N: CD45RA⁺, CD27⁺), central memory (CM: CD45RA⁻, CD27⁺), effector memory (EM: CD45RA⁻, CD27⁻), and terminally differentiated late effector cells (TEMRA: CD45RA⁺, CD27⁻). (B) Total counts of γδ T-cell subsets after 7 days stimulation with iDC^Zol− and iDC^Zol+. Bars represent the mean ± SEM of 5 experiments. Differences between CM and EM γδ T cells are statistically significant (CM γδ T cells: iDC^Zol− 3600 ± 1400 versus iDC^Zol+ 54 000 ± 20 000; P < .03; EM γδ T cells: iDC^Zol− 4700 ± 2100 versus iDC^Zol+ 28 000 ± 11 000; P < .04). (C) Expression of homing receptors (CD62L) and costimulatory molecules (HLA-DR, CD80) on the surface of γδ T cells after stimulation with iDC^Zol+. Results are from 1 of 5 experiments. (D) Total counts of viable CD62L⁺, CD80⁺, and HLA-DR⁺ γδ T cells after stimulation with iDC^Zol+. Bars represent the mean (± SEM) of 5 experiments. Total counts of viable CD62L⁺ γδ T cells per well are significantly increased (CD62L⁺ γδ T cells: iDC^Zol− 13 500 ± 5300 versus iDC^Zol+ 62 000 ± 9000; P < .005; HLA-DR⁺ γδ T cells: iDC^Zol− 12 000 ± 4000 versus iDC^Zol+ 78 400 ± 21 000; P > .05; CD80⁺ γδ T cells: iDC^Zol− 4000 ± 1200 versus iDC^Zol+ 36 000 ± 16 000; P > .05).