Proliferative expansion of γδ T cells by iDC^Zol+, mDC^Zol+, and Mo^Zol+. (A) Flow cytometry of representative γδ T-cell expansions after 7 days stimulation with iDC^Zol+ (upper quadrants), mDC^Zol+ (middle quadrants), and Mo^Zol+ (lower quadrants). mDC^Zol+ were matured with TNF-α and IL-1β. Results are from 1 representative out of 11 (iDC) to 4 (Mo) experiments. (B) Total counts of viable cells, γδ T cells, and αβ T cells per well after 7 days of stimulation with different iDCs, mDCs, and Mo subsets. Bars represent the mean (± SEM) of 11 (iDCs) to 4 (mDCs) experiments. Side-by-side experiments (n = 4) and unpaired experiments are pooled together. Total counts of γδ T cells are significantly higher in iDC^Zol+ versus iDC^Zol− (133 000 ± 44 000 versus 14 000 ± 4200; n = 11, P < .001), mDC^Zol+ versus mDC^Zol− (124 000 ± 41 000 versus 21 700 ± 6000; n = 6, P < .005), and Mo^Zol+ versus Mo^Zol− (104 000 ± 40 000 versus 21 000 ± 9000; n = 4, P < .05). When the comparison is restricted to side by side experiments, differences are not statistically different between iDC^Zol+ (285 000 ± 96 000 γδ T cells/well), mDC^Zol+ (250 000 ± 67 000 γδ T cells/well) and Mo^Zol+ (103 000 ± 40 000 γδ T cells/well). Differences among total counts of αβ T cells are also not statistically different (iDC^Zol−: 208 000 ± 36 000 αβ T cells/well; iDC^Zol+: 188 000 ± 33 000 αβ T cells/well; mDC^Zol−: 189 000 ± 43 000 αβ T cells/well, mDC^Zol+: 177 000 ± 33 000 αβ T cells/well; Mo^Zol−: 152 000 ± 54 000 αβ T cells/well, Mo^Zol+: 146 000 ± 39 000 αβ T cells/well). P > .05).