Workflow of the screen for inhibitors of the cyclin D2 promoter. Hits from the high-throughput screen were assessed for reproducibility and selectivity for c-maf. Of the 31 c-maf–dependent inhibitors identified, 24 were unique to one of the libraries tested, and 7 were detected in the screens of both libraries. Of the 8 c-maf–independent inhibitors, 7 were unique to one of the libraries, and 1 was identified in both screens.