Figure 2
Figure 2. Cell adhesion–mediated p27Kip1 protein levels are posttranslationally regulated through down-regulation of the SCFSkp2 ubiquitin ligase Skp2. (A) An RPA for gene expression of p27Kip1, p21, and other cell-cycle–related molecules in Jeko-1 and SUDH-10 cells adhered to HS-5 versus those placed in suspension media. (B) The p27Kip1 and Skp2 expression in Jeko-1 cells in suspension (Sus) versus HS-5 adhesion (HS5-Ad) analyzed by Western blot. Cell-cycle distribution (C) and p27Kip1 and Skp2 expression (D) 24 hours after transfection with either Skp2 siRNA (siSkp2) or control siRNA (siCont) in Jeko-1 cells in suspension. (E) Cyclin D1 expression in Jeko-1 and SUDH-10 lymphoma cells in suspension (Sus) versus HS-5 adhesion (HS5-Ad) analyzed by Western blot. All data are representative of at least 3 experiments (means ± SD).

Cell adhesion–mediated p27Kip1 protein levels are posttranslationally regulated through down-regulation of the SCFSkp2 ubiquitin ligase Skp2. (A) An RPA for gene expression of p27Kip1, p21, and other cell-cycle–related molecules in Jeko-1 and SUDH-10 cells adhered to HS-5 versus those placed in suspension media. (B) The p27Kip1 and Skp2 expression in Jeko-1 cells in suspension (Sus) versus HS-5 adhesion (HS5-Ad) analyzed by Western blot. Cell-cycle distribution (C) and p27Kip1 and Skp2 expression (D) 24 hours after transfection with either Skp2 siRNA (siSkp2) or control siRNA (siCont) in Jeko-1 cells in suspension. (E) Cyclin D1 expression in Jeko-1 and SUDH-10 lymphoma cells in suspension (Sus) versus HS-5 adhesion (HS5-Ad) analyzed by Western blot. All data are representative of at least 3 experiments (means ± SD).

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