Isolated hESC-derived endothelial cells (PECAM1⁺ cells). The cells were grown in culture for up to 7 passages. Cells are stained with VE-cad (original magnification, × 1000; 100×/1.40 oil-immersion objective lens) and VWF (original magnification, × 400; 40×/1.3 objective lens) and show the uptake of Dil-labeled acetylated low-density lipoprotein (Dil-Ac-LDL; original magnification, × 200; 20×/0.5 NA air objective lens). Micrographs were taken at 25°C using a fluorescent motorized microscope (Axiovert 200; Carl Zeiss, Oberkochen, Germany) equipped with an Orca digital camera (Hamamatsu Photonics, Tokyo, Japan). OpenLab (ImproVision, Coventry, United Kingdom) and Fluoromount were used as imaging software and medium, respectively.