No detectable effect of Ba103 treatment on type I or IV hypersensitivity reaction. (A) BALB/c mice were treated with an intravenous injection of 30 μg Ba103 or control IgG. A day later, the mice were passively sensitized with an intradermal injection of TNP-specific IgE (■) or PBS (▩) into the ear, followed by the intravenous injection of TNP-OVA plus Evans blue dye the next day. The extravasated dyes were extracted from the ear skin 30 minutes after challenge, and the amount of dye was measured by spectrophotometry. (B) BALB/c mice were epicutaneously sensitized with hapten DNFB on the abdomen; 5 days later, they were challenged epicutaneously with DNFB on the left ear and with vehicle alone on the right ear. The mice were treated with 2 intravenous injections of 30 μg Ba103 each, the first 1 day before the sensitization and the second 1 day before the antigen challenge (○). As a control, the other group of mice was treated with the same amount of control IgG in place of Ba103 (■). The thicknesses of the left and right ears were measured at the indicated time points after the antigen challenge. The values of ΔEar thickness, the differences in ear thickness (left-right) at each time point, are plotted. Data are expressed as the mean (± SEM) of 3 mice in each group and are representative of 3 repeated experiments.