Time course of the basophil depletion in Ba103-treated mice. BALB/c mice were treated with an intravenous injection of 30 μg Ba103 (○) or control rat IgG (■). At the indicated time points after the injection, spleen cells were isolated and stained for FcϵRIα and CD49b to identify and count the basophils. The relative number of basophils in spleen after the injection of Ba103 (○) or control IgG (■) over time is shown. The cell numbers on day 0 (before the treatment) were set as 100%. Data are expressed as the mean (± SEM) of 5 mice in each group and are representative of 3 repeated experiments.