The phosphorylation of ERK1/2 is intact in Pla2g5-null BMMCs stimulated with IgE and antigen or SCF. (A-B) BMMCs from wild-type (shaded bars) and Pla2g5-null (open bars) mice were sensitized with IgE anti-TNP and stimulated with 100 ng/mL TNP-BSA or buffer alone for 10 minutes (A) or were stimulated for 10 minutes with 100 ng/mL SCF or buffer alone (B). Supernatants were assayed for LTC₄ (left) and PGD₂ (right) by EIA. The values are the mean ± SEM of 2 independent experiments with 3 separate cultures of each strain of BMMCs (n = 3). (C-D) BMMCs from wild-type (left) and Pla2g5-null (right) mice were stimulated with IgE and antigen (C) or with SCF (D) for up to 60 minutes. Pellets were analyzed for phosphorylated (p) and total ERK-1/2 and cPLA₂α by Western blotting.