Activation of ERK and cPLA₂α and the ensuing LTC₄ and PGD₂ generation by BMMCs in response to Pam₃CSK₄ require TLR2 and MyD88. (A) BMMCs derived from Myd88-null (□) and wild-type C57BL/6 (■) mice were incubated for 60 minutes with the indicated concentrations of Pam₃CSK₄ for 60 minutes. The supernatants were assayed for LTC₄ (left panel) and PGD₂ (right panel) by EIA. (n = 3). Error bars indicate SEM. (B) BMMCs derived from Tlr2-null (n = 3), Myd88-null (n = 3), and wild-type C57BL/6 (n = 4) mice were incubated with 3 μg/mL Pam₃CSK₄ in a time-dependent manner. Pellets were analyzed for phosphorylated (left panels) or total (right panels) ERK and cPLA₂α by Western blotting.