Defective fetal liver definitive erythropoiesis in palladin-deficient embryos. (A) Typical images of wt and Palld^−/− E13.5 fetal livers. (B) The cellularity of wt and Palld^−/− E13.5 fetal livers. Error bar represents plus and minus a SD; **P < .01. (C) H&E staining of wt and Palld^−/− fetal liver sections. (D) TUNEL assay shows increased apoptosis in Palld^−/− fetal liver at the same location as in panel C. Note that the apoptosis mainly occurs in the region where hematopoiesis takes place (arrows). (C,D) Images were viewed with an Olympus BX51 microscope with an Olympus UPlanFl 10×/0.30 objective, captured with a SPOT RTKE cooled color CCD camera (Diagnostic Instruments), and imported into SPOT software (Diagnostic Instruments). In panel D, DAPI (nuclei) and FITC (apoptotic cells) fluorescence are shown. Original magnification, × 100.