Analysis of chimeric mice generated by liver transplantation from WAVE1^−/− embryos. (A) EDTA-treated peripheral blood was obtained from live chimeric mice. (B) Washed platelets from WAVE1^+/+, WAVE1^+/−, or WAVE1^−/− mice were plated onto fibrinogen-coated coverslips for 45 minutes in the presence of apyrase (2 U/mL) and indomethacin (10 μM) with or without 1 U/mL thrombin. Some preparations included 100 nM PMA, 10 μg/mL collagen I, or 100 μM ADP. Cells were fixed, permeabilized, and stained with rhodamine-conjugated phalloidin to mark F-actin and analyzed by confocal microscopy (see “Image acquisition for platelet and MK spreading” for complete image acquisition information. Scale bar equals 15.8 μm. This experiment is representative of 4 thus performed (from 2 independent transplantations). (C) Platelet spreading was quantified by computer analysis of platelet surface areas. Each bar represents at least 100 platelets analyzed as described in “Image acquisition for platelet and MK spreading.” The results shown are means (± SD).