Expression and distribution of WAVE1 and WAVE2 in fibrinogen- and collagen-adherent MKs derived from ES cells. On day 12 of the differentiation protocol, MKs were plated onto 100 μg/mL fibrinogen, or onto 10 μg/mL collagen I, and allowed to stand for 60 minutes. Cells were fixed, permeabilized, and stained with rhodamine-conjugated phalloidin to mark F-actin (red) and with anti-WAVE1 or anti-WAVE2 antibodies followed by Alexa 488 (green), and analyzed by confocal microscopy. Scale bar equals 10 μm. See “Image acquisition for platelet and MK spreading” for complete image acquisition information.