VEGF inhibits thymic T-cell development via VEGFR-2. (A) The representative thymuses from PBS, VEGF-A, VEGFR2-sel, and VEGFR1-sel mice (from left to right). (B) The total thymocytes. (C) Flow cytometric analysis of thymocytes expressed CD4 and CD8a in CD3e^low/− cells. The representative FACS plots were shown. The first plot is the isotype control. (D) The total numbers of T-cell subtypes in thymus. All of the data (mean ± SEM, n ≥ 3) repeat 3 times. *P < .05; **P < .01.