VEGF decreases splenic T cells via VEGFR-2 and VEGFR-1 slightly decreases the inhibition of VEGFR-2. Age-matched Balb/c mice were given PBS or VEGF receptor agonists for 28 days as described in Figure 1. (A) Flow cytometric analysis of the expression of CD4 and CD8a in splenocytes. The representative FACS plots were shown. The first plot is the isotype control. (B) The total numbers of T-cell subsets in spleens. The data (mean ± SEM, n ≥ 3) repeat 3 times. (C) Balb/c mice were given PBS treated with rat IgG, or rhVEGF-treated rat IgG, anti-R1, or anti-R2 as described in Figure 1C. The total numbers of T-cell subsets in spleen were shown. The data (mean ± SEM, n ≥ 5) repeat 2 times. *P < .05; **P < .01.