American Society of Hematology

Figure 1

$Figure 1. GSK-3β accumulates in the nucleus of CLL B cells. (A) Equivalent amounts (50 μg) of nuclear and cytosolic proteins isolated from the indicated sample from patients with CLL and from normal human B cells were separated by SDS-PAGE, immunoblotted, and probed with antibodies to the indicated proteins. P = patient. Cu/Zn super oxide dismutase (Cu/Zn SOD) and ORC2 are used as markers for the purity of the cytoplasmic and nuclear proteins, respectively. (B) Nuclear/cytosolic fractions were prepared from the indicated samples from patients with CLL, and protein expression was analyzed as described in panel A. (C) Immunofluorescence staining of GSK-3β (probed with TRITC-labeled anti-mouse secondary antibody, red fluorescence) in CLL B cells (top) and normal human B cells (bottom). Nuclei were counterstained with Hoechst 33342 (blue fluorescence).$

GSK-3β accumulates in the nucleus of CLL B cells. (A) Equivalent amounts (50 μg) of nuclear and cytosolic proteins isolated from the indicated sample from patients with CLL and from normal human B cells were separated by SDS-PAGE, immunoblotted, and probed with antibodies to the indicated proteins. P = patient. Cu/Zn super oxide dismutase (Cu/Zn SOD) and ORC2 are used as markers for the purity of the cytoplasmic and nuclear proteins, respectively. (B) Nuclear/cytosolic fractions were prepared from the indicated samples from patients with CLL, and protein expression was analyzed as described in panel A. (C) Immunofluorescence staining of GSK-3β (probed with TRITC-labeled anti-mouse secondary antibody, red fluorescence) in CLL B cells (top) and normal human B cells (bottom). Nuclei were counterstained with Hoechst 33342 (blue fluorescence).

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