Hypermethylation of Wnt inhibitors is associated with up-regulation of genes implicated in the Wnt-signaling pathway in ALL patients. Expression of the Wnt pathway genes in ALL patients was measured by qRT-PCR. Significantly higher levels of WNT16 (A), FZ3 (B), TCF1 (C), LEF1 (D), and cyclin D1 (E) transcripts were detected among methylated ALL patients compared with unmethylated patients. Bars represent the mean expression (95% CI) in patients with ALL in comparison with healthy lymphocytes (normalized ratio = 100%).