Methylation of Wnt inhibitors is associated with activation of Wnt-signaling pathway in ALL. (A-B) Expression of Wnt-signaling pathway genes before and after treatment with the demethylating agent 5-aza-2′-deoxycytidine (Aza) in comparison with healthy lymphocytes (normalized ratio = 100%) in TOM-1 (A) and NALM-20 (B) ALL-derived cell lines. The mean ± SD of 3 different experiments is shown. (C) Subcellular location of β-catenin in the ALL cell line TOM-1. β-Catenin is located preferentially in the nucleus indicating activation of the Wnt pathway in ALL. Treatment with 5-aza-2′-deoxycytidine decreases the nuclear location of β-catenin. A representative example of 3 experiments is shown.