Methylation and expression analysis of Wnt-antagonist genes in ALL-derived cell lines. (A) MSP analysis of the methylated sequences in different ALL-cell lines. (B-C) Expression of Wnt inhibitors in TOM-1 (B) and NALM-20 (C) cell lines before and after treatment with the demethylating agent 5-aza-2′-deoxycytidine (Aza) demonstrating an up-regulation of gene expression after treatment. Gene expression was normalized with expression in normal lymphocytes (normalized ratio = 100%). The mean ± SD of 3 different experiments (B-C) is shown.