Differentiation potential of the cells expanded following 5azaD/TSA treatment in the ex vivo culture. After 9 days of initial culture (as described in “Material and methods”), 5azaD/TSA-treated cells were placed in a secondary culture supplemented with GM-CSF, G-CSF, SCF, IL-3, IL-6, and EPO in the absence of additional 5azaD/TSA treatment. Cytospin preparations were stained with Giemsa and Wright stains and viewed with a light microscope (objective used 40×/0.75 NA) equipped with an Axiocam camera (Zeiss, Thornwood, NY). Images were processed using Zeiss Axiovision software version 4.1. The phenotype of cells was determined by staining with mAb directed toward CD34, CD90, CD14, CD15, CD36, and lineage markers (CD2, CD14, CD15, CD19, glycophorin A).