DC_regs suppress cytokine production in the transplanted recipient mice. The irradiated recipient BALB/c mice received T-cell-depleted BM cells and CD4⁺CD25⁻ T cells obtained from B10.D2 mice, and were then treated with or without the recipient-type mDCs, DC_regs, or RAPA after the transplantation as described in Figure 3A,B. Subsequently, serum was collected from each recipient mouse 30 days after the transplantation. Serum production of TNF-α (A), IL-12p40 (B), IFN-γ (C), and IL-4 (D) was measured by ELISA. The error bars are SD. *P < .01 compared with the untreated group by Student paired t test. Results of 3 replicated experiments were pooled.