T-cell reconstitution in nonirradiated lymphoid cell–deficient mice by LSK cells cultured on immobilized Delta1^ext-IgG compared with noncultured LSK cells. Nonirradiated Ly5.1 Rag^−/− recipients received 1 × 10³ noncultured LSK cells or 1 × 10⁶ Ly5.2 Delta1^ext-IgG-cultured cells. FACS analysis and CBCs were performed on blood samples obtained at various times after HCT to determine the number of Ly5.2⁺ CD3⁺ cells in mice that received LSK cells cultured on Delta (▪) compared with noncultured LSK cells (▵). Data represent the mean plus or minus SEM of 3 separate experiments (n = 5-10)