ABT-869 inhibits phosphorylation of FLT3, STAT5, and ERK in MV-411 cells. MV-4-11 cells were cultured in the presence of varying concentrations of ABT-869 or vehicle control (0.1% DMSO) for 2 hours. Cells were lysed and lysates analyzed by Western blot using (A) anti–phospho-FLT3, (B) anti–phospho-STAT5, (C) anti–phospho-ERK for MV-4-11 cells, and (D) anti–phospho-STAT5 and anti–Pim-1 for MOLM-13 cells. For cell culture, β-actin was used as the load control for FLT3 and STAT5, whereas total ERK was used for ERK. The results from at least 2 independent experiments demonstrate similar inhibition of FLT3, STAT5, and ERK phosphorylation with ABT-869, a representative blot is shown.