Figure 3
Failure of Pten-deficient immature Vα14iNKT cells to mature in vitro. CD44highNK1.1− (stage 2, immature) Vα14iNKT cells were purified from the thymi of Ptenflox/flox or LckCrePtenflox/flox mice and injected into FTOCs derived from a Jα18−/− embryo. (Left) Flow cytometric analysis of cell populations injected. (Right) At 9 days after injection, Vα14iNKT cells in the FTOC were isolated and analyzed by flow cytometry to detect stage 3 (mature) cells. Mature Vα14iNKT cells were very rare in FTOCs receiving Pten-deficient stage 2 NKT cells. One result representative of 3 experiments is shown.

Failure of Pten-deficient immature Vα14iNKT cells to mature in vitro. CD44highNK1.1− (stage 2, immature) Vα14iNKT cells were purified from the thymi of Ptenflox/flox or LckCrePtenflox/flox mice and injected into FTOCs derived from a Jα18−/− embryo. (Left) Flow cytometric analysis of cell populations injected. (Right) At 9 days after injection, Vα14iNKT cells in the FTOC were isolated and analyzed by flow cytometry to detect stage 3 (mature) cells. Mature Vα14iNKT cells were very rare in FTOCs receiving Pten-deficient stage 2 NKT cells. One result representative of 3 experiments is shown.

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