Pten gene deletion in NKT cells in LckCrePten^flox/flox mice. (A) Genomic DNA from TCRβ⁺NK1.1⁺HSA^low thymocytes of the indicated genotype was analyzed by PCR. In LckCrePten^flox/flox mice, most TCRβ⁺NK1.1⁺HSA^low thymocytes contained the deleted Pten allele (Δ) rather than the WT Pten allele (flox). (B) Western blot analysis of Pten protein expression by the thymocytes in panel A. Actin loading control.