Western blot analysis of blood cell extracts. Immunoblot analysis of (A) erythrocyte and (B) total leukocyte lysates of healthy controls as well as of patient 1a, patient 2, and patient 2's parents. All samples were loaded on the same gel, allowing direct comparison. They are shown separately for clarification. Band-3 and β-actin served as loading controls. Note the faint GR staining in patient 2's leukocyte lysate.