American Society of Hematology

Figure 4

$PDC-induced IL-10 is almost exclusively produced by CD4+CD45RA− T cells. CD4+ T cells were immunomagnetically separated into CD45RA+ and CD45RA− fractions, combined with PDCs, and then compared as to SEB-induced cytokine production at 36 hours by 2 different methods. (A) IL-10 and IFN-γ were measured with the cytokine secretion assay. One of 2 similar experiments is shown. (B) IL-10 was measured in the supernatants with CBA, and the contribution of IL-10 from each subset in percentage of total IL-10 was calculated, taking into account the relative frequency of the 2 cell populations. Two separate experiments are shown, as indicated by distinct symbols.$

PDC-induced IL-10 is almost exclusively produced by CD4⁺CD45RA⁻ T cells. CD4⁺ T cells were immunomagnetically separated into CD45RA⁺ and CD45RA⁻ fractions, combined with PDCs, and then compared as to SEB-induced cytokine production at 36 hours by 2 different methods. (A) IL-10 and IFN-γ were measured with the cytokine secretion assay. One of 2 similar experiments is shown. (B) IL-10 was measured in the supernatants with CBA, and the contribution of IL-10 from each subset in percentage of total IL-10 was calculated, taking into account the relative frequency of the 2 cell populations. Two separate experiments are shown, as indicated by distinct symbols.

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