MLN3897 abrogates CCL3-induced migration by inhibiting Akt phosphorylation. Effects of MLN3897 on cell migration in the presence of CCL3 (5 ng/mL) were assessed by Transwell migration assay. MM cells were preincubated with MLN3897 (10 nM for 4 hours) (A) or PI3K inhibitor LY94002 (25 μM for 1 hour) (C), and then seeded on the upper chamber of the Transwell plate. After 4 hours, cells that migrated to the lower chamber were counted, and results were expressed as fold increase over control plus or minus standard deviation. (B) CCL3 (100 ng/mL for 30 minutes) stimulated Akt phosphorylation in serum-starved (overnight in 1% and 30 minutes in 0% RPMI media) MM cells, assessed by Western blot analysis. In selected experiments, cells were preincubated with MLN3897 for 4 hours. The densitometric analysis of the immunoblots displays partial inhibition of Akt phosphorylation by MLN3897 in MM.1S and complete inhibition in OPM1 cells. Results are expressed as mean (± SD).