Figure 3
CCR1 expression and CCL3 secretion by MM cell lines and primary MM cells. (A) CCR1 expression on MM cell lines and patient MM cells was analyzed by flow cytometry. A representative flow cytometry shows percentage of positive cells from at least 3 different experiments. CCR1 expression on monocytes is shown as a positive control. (B) CCR5 expression on MM1.S, OPM1, INA6, and U266 was analyzed. Flow cytometry data represent several independent experiments. (C) CCL3 secretion was assessed by ELISA on 48-hour culture supernatants of MM cell lines, MM primary cells, and monocytes plated at a density of 106 cells/mL. Means (± SD) are expressed as pg/mL on a logarithmic scale.

CCR1 expression and CCL3 secretion by MM cell lines and primary MM cells. (A) CCR1 expression on MM cell lines and patient MM cells was analyzed by flow cytometry. A representative flow cytometry shows percentage of positive cells from at least 3 different experiments. CCR1 expression on monocytes is shown as a positive control. (B) CCR5 expression on MM1.S, OPM1, INA6, and U266 was analyzed. Flow cytometry data represent several independent experiments. (C) CCL3 secretion was assessed by ELISA on 48-hour culture supernatants of MM cell lines, MM primary cells, and monocytes plated at a density of 106 cells/mL. Means (± SD) are expressed as pg/mL on a logarithmic scale.

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