![Figure 5. Epinephrine-treated SS RBCs adhere to Lu-Fc in an integrin α4β1–dependent manner under flow conditions. (A) AA and SS RBCs (.005 [0.5%] hematocrit) were either untreated or incubated with 20 nM epinephrine (Epi) for 1 minute at room temperature, then injected into a Lu-Fc–coated microslide (0.3 mg/mL) at 0.2 dyne/cm2 for 10 minutes before applying 5-minute washout steps at 0.4, 1, 2, and 4 dyne/cm2. Cells were counted after each wash in 6 areas along the microslide centerline. (B) AA (AA1 and AA2), SS (SS1-SS6), and high-reticulocyte control (C1-C3) RBC samples (.005 [0.5%] hematocrit) were either untreated or incubated with 20 nM epinephrine with or without preincubation with 10 μg/mL blocking antibody anti–integrin β1 (mAb 13), or with 1 μg/mL activating antibody anti–integrin β1 TS2/16. Injection and washouts were carried out as for panel A. The graph shows results at 1 dyne/cm2. Each value is a mean of 6 measures. (**P < .01; in panel B, ▧ and ▪ are compared with □, and ⊡ is compared with ▪). Error bars represent SD.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/109/8/10.1182_blood-2006-07-035139/4/zh80080711120005.jpeg?Expires=1724845770&Signature=veSYIZ~LbowFonFi7~Fx9XWaoNF2rKvJieSG3vyTWqQoFRjEGmhv94IWephN-MRis-9-nI5aeHmzTuU~CvYa1sFB6aeTPTSMqb9iQtnFANHZu8-Q7X2Jt0iap9jb6PKtysPg8pQN1cjUii5FuJBoCQVugC6ISO~0olHOFCgGPW9sNF3TsmjgYaUPyYeZ1EIUci2yAe8scArl3CAovc~G4R3B0~KahgWEn9rQ0JJF1CnHsMeV9gJ-XD64h8qQbrEjZJt1iVDsJuVO4x0HklwAsfZbkwMwN4CbjI9wEdxCepN7Y5bKgtzJMSv34x3GvmLLE0hTjLMEmgxRvaU6DXoUBg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Epinephrine-treated SS RBCs adhere to Lu-Fc in an integrin α4β1–dependent manner under flow conditions. (A) AA and SS RBCs (.005 [0.5%] hematocrit) were either untreated or incubated with 20 nM epinephrine (Epi) for 1 minute at room temperature, then injected into a Lu-Fc–coated microslide (0.3 mg/mL) at 0.2 dyne/cm2 for 10 minutes before applying 5-minute washout steps at 0.4, 1, 2, and 4 dyne/cm2. Cells were counted after each wash in 6 areas along the microslide centerline. (B) AA (AA1 and AA2), SS (SS1-SS6), and high-reticulocyte control (C1-C3) RBC samples (.005 [0.5%] hematocrit) were either untreated or incubated with 20 nM epinephrine with or without preincubation with 10 μg/mL blocking antibody anti–integrin β1 (mAb 13), or with 1 μg/mL activating antibody anti–integrin β1 TS2/16. Injection and washouts were carried out as for panel A. The graph shows results at 1 dyne/cm2. Each value is a mean of 6 measures. (**P < .01; in panel B, ▧ and ▪ are compared with □, and ⊡ is compared with ▪). Error bars represent SD.
